Oakley at 20:06, 15 November 2011

2011-11-15T20:06:18Z

Oakley: Created page with "Use gloves! 50mL buffer ~0.5g agarose 1 uL ethidium bromide Mix agarose into buffer and heat until all agarose is incorporated. Wait until mixture is cool enough to hold comfor…"

2011-11-15T20:05:45Z

Created page with "Use gloves! 50mL buffer ~0.5g agarose 1 uL ethidium bromide Mix agarose into buffer and heat until all agarose is incorporated. Wait until mixture is cool enough to hold comfor…"

New page

Use gloves!

50mL buffer
~0.5g agarose
1 uL ethidium bromide

Mix agarose into buffer and heat until all agarose is incorporated.
Wait until mixture is cool enough to hold comfortably in hand then add ethidium bromide
Rotate glass jar gently then cool
Place rubber stoppers on the ends of the plastic channel
Insert comb to form wells, rounded edges up
Make sure the combs are at least 1mm away from the bottom
Pour agarose mixture into channel
Pour buffer into dish below the gel channel
Wait until cool (gel will become cloudy)
Place Ladder into a well as a standard size (5uL)
Mix loading buffer (1 uL) with the sample you are running (total ~5uL) on parafilm and load
Turn meter on to ~100 Volts
Align channel so that the + side is farther from the samples
Let run until sample bands are about 1/2 way down the gel

@@ Line 5: / Line 5: @@
 uL ethidium bromide
-Mix agarose into buffer and heat until all agarose is incorporated.
+# Mix agarose into buffer and heat until all agarose is incorporated.
-Wait until mixture is cool enough to hold comfortably in hand then add ethidium bromide
+# Wait until mixture is cool enough to hold comfortably in hand then add ethidium bromide
-Rotate glass jar gently then cool
+# Rotate glass jar gently then cool
-Place rubber stoppers on the ends of the plastic channel
+# Place rubber stoppers on the ends of the plastic channel
-Insert comb to form wells, rounded edges up
+# Insert comb to form wells, rounded edges up
-Make sure the combs are at least 1mm away from the bottom
+# Make sure the combs are at least 1mm away from the bottom
-Pour agarose mixture into channel
+# Pour agarose mixture into channel
-Pour buffer into dish below the gel channel
+# Pour buffer into dish below the gel channel
-Wait until cool (gel will become cloudy)
+# Wait until cool (gel will become cloudy)
-Place Ladder into a well as a standard size (5uL)
+# Place Ladder into a well as a standard size (5uL)
-Mix loading buffer (1 uL) with the sample you are running (total ~5uL) on parafilm and load
+# Mix loading buffer (1 uL) with the sample you are running (total ~5uL) on parafilm and # load
-Turn meter on to  ~100 Volts
+# Turn meter on to  ~100 Volts
-Align channel so that the + side is farther from the samples
+# Align channel so that the + side is farther from the samples
-Let run until sample bands are about 1/2 way down the gel
+# Let run until sample bands are about 1/2 way down the gel

Pouring an agarose gel - Revision history

Oakley at 20:06, 15 November 2011

Oakley: Created page with "Use gloves! 50mL buffer ~0.5g agarose 1 uL ethidium bromide Mix agarose into buffer and heat until all agarose is incorporated. Wait until mixture is cool enough to hold comfor…"