The Data Dump Paper - Revision history

Federico.gaiti: /* Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline */

2012-01-16T18:00:07Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-25T14:23:30Z

2011-12-25T14:19:12Z

2011-12-23T16:37:35Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-23T16:37:10Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-23T16:36:46Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-23T16:36:12Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-23T10:50:21Z

‎No evidence that Ptx ablates Hydra photobehavior

2011-12-23T10:04:48Z

‎Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline

2011-12-05T10:05:34Z

@@ Line 68: / Line 68: @@
 * In bright light (and Nana-proline), the Gi inhibitor Ptx seems to have no effect (could formally test that slope of Ptx concentration and firing rate is NSD than zero)
-* In dim light (and Nana-proline), the Gi inhibitor Ptx affects firing rate.  More ptx yields less firing. This is consistent with Watson's results above, where light intensity was not specified (it was probably not as bright as our 3500 lux trial). Watson suggested that proline inhibits firing through Gi. This does not explain how proline increases firing compared to ASW alone, as Fede found, and Watson himself found for Contact Sensitive Firing.
+* In dim light (and Nana-proline), the Gi inhibitor Ptx affects firing rate.  More ptx yields more firing. This is consistent with Watson's results above, where light intensity was not specified (it was probably not as bright as our 3500 lux trial). Watson suggested that proline inhibits firing through Gi. This does not explain how proline increases firing compared to ASW alone, as Fede found, and Watson himself found for Contact Sensitive Firing.
 ==Discussion==

@@ Line 53: / Line 53: @@
 In all trials across various Ptx concentrations where the Hydra remained responsive to mechanical stimuli, they also remained responsive to bright lights.
-[[File:FedeHydra2.jpg|600px]]
+[[File:ProportionResponsiveContractedHydra.jpeg|600px]]
 [[Fede Hydra Scrunch Ptx]]

@@ Line 49: / Line 49: @@
 Some combinations of Ptx concentration and duration of exposure cause Hydra to be unresponsive to probing. Usually, they will scrunch into a ball upon probing in a behavior that looks the same as exposure to bright light.
-[[File:FedeHydra1.jpg]]
+[[File:ProportionUnresponsiveHydra.jpeg]]
 In all trials across various Ptx concentrations where the Hydra remained responsive to mechanical stimuli, they also remained responsive to bright lights.

@@ Line 61: / Line 61: @@
 === Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline===
-[[File:FedeHali.jpg|600px]]
+[[File:FedeHali.jpg|700px]]
 [[Fede Haliplanella Raw Data]]

@@ Line 61: / Line 61: @@
 === Influence of Ptx (Gi inhibitor) in the presence of NANA and Proline===
-[[File:FedeHali.jpg|350px]]
+[[File:FedeHali.jpg|600px]]
 [[Fede Haliplanella Raw Data]]

← Older revision		Revision as of 10:05, 5 December 2011
Line 71:		Line 71:

	==Discussion==		==Discussion==
		+	* Proline and NANA, by themselves, invoke biphasic responses (Watson and Hessinger 1992) - at low concentrations, but not high concentrations, they act as stimulants.
		+	* Fede's data shows that Proline and NANA can cancel each other out! There is thus an integrator that takes into account Proline + NANA.
		+	* In dim light: Ptx stops NANA + Proline (NP) from being an inhibitor; however, it doesn't stop them from being a stimulant . . . this means that Proline and/or NANA are acting through both a Gs pathway and a Gi pathway (or, more accurately, either or both can act as both an inhibitor and stimulant).
		+	* In bright light: inhibition by light overwhelms stimulation by Proline and/or NANA.
		+	* The big question is how you get biphasic responses (as observed by Watson and Hessinger 1992 for Haliplannela): is it 1) a matter of pathway exhaustion (in which case the response is duration times ligand concentration) or 2) competing receptors w/ different binding affinities (in which case the response depends entirely on ligand concentration).