Difference between revisions of "Glycerol Stocks"

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| class="unsortable" align="center" style="background:#f0f0f0;"|'''Notes'''
 
| class="unsortable" align="center" style="background:#f0f0f0;"|'''Notes'''
 
| class="unsortable" align="center" style="background:#f0f0f0;"|'''Sequence Files'''
 
| class="unsortable" align="center" style="background:#f0f0f0;"|'''Sequence Files'''
−
| class="unsortable" align="center" style="background:#f0f0f0;"|'''Primers Used'''
+
| class="unsortable" align="center" style="background:#f0f0f0;"|'''Primers Used for Seq'''
 
| align="center" style="background:#f0f0f0;"|'''Lab Member'''
 
| align="center" style="background:#f0f0f0;"|'''Lab Member'''
 
|-
 
|-
−
| Vt10||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/20-Vt10F_D03_025.seq][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/21-Vt10R_E03_023.seq]||1F/6R||Nicole
+
| Vt10||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/20-Vt10F_D03_025.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/21-Vt10R_E03_023.seq R]||1F/6R||Nicole
 
|-
 
|-
−
| Vt12||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/22-Vt12F_F03_021.seq][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/23-Vt12R_G03_019.seq]||1F/6R||Nicole
+
| Vt12||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/22-Vt12F_F03_021.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/23-Vt12R_G03_019.seq R] , [http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/73-Vt12F_A10_080.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/74-Vt12R_B10_078.seq R]||1F/6R , 19/20||Nicole
 
|-
 
|-
−
| Vt13||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/24-Vt13F_H03_017.seq][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/25-Vt13R_A04_032.seq]||1F/6R||Nicole
+
| Vt13||10/12/2011||V. tsujii luciferase gene inserted in pcR2.1 vector; reversed||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/24-Vt13F_H03_017.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/25-Vt13R_A04_032.seq R]||1F/6R||Nicole
 
|-
 
|-
−
| pcDNA3||10/21/2011||pcDNA3 aliquot from Nick Doerr (Weimb's Lab)||Nicole
+
| pcDNA3||10/21/2011||pcDNA3 aliquot from Nick Doerr (Weimb's Lab)||||||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 1||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||Nicole
+
| Vt12/pcDNA3 1||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/89-vt1-F1_A12_096.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/90-vt1-R1_B12_094.seq R]||19/20||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 2||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||Nicole
+
| Vt12/pcDNA3 2||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/91-vt2-F1_C12_092.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/92-vt2-R1_D12_090.seq R]||19/20||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 3||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||Nicole
+
| Vt12/pcDNA3 3||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/93-vt3-F1_E12_088.seq F][http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/94-vt3-R1_F12_086.seq R]||19/20||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 4||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert ||Nicole
+
| Vt12/pcDNA3 4||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert ||||||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 5||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert ||Nicole
+
| Vt12/pcDNA3 5||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert ||||||Nicole
 
|-
 
|-
−
| Vt12/pcDNA3 6||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||Nicole
+
| Vt12/pcDNA3 6||12/9/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed ||||||Nicole
−
|
+
|-
 +
| vt12_6||12/16/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; correct||||||Nicole
 +
|-
 +
| Ligation 8/15 PhyVec, Ligation 8/15 Regrowth||8/15/2011||pHyVec1 derived plasmid: GFP, actin promoter, and actin polyadenylation site from hoTG trimmed and blunted at NsiI sites and cloned into Bluescript blunted at KpnI and SacI sites of MCS ||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/pHyVec13.txt pHyVec13]||||Federico
 +
|-
 +
| pCR4-SpoCRY1-3||4/26/2012||Amp selection||||||Nicole
 +
|-
 +
| pMalC2-SpoCRY1-3||4/26/2012||Amp selection; for expression in BL21 or DH5a; MBP tagged||||||Nicole
 +
|-
 +
| pAC5.1-SpoCRY2.V5H||4/26/2012||Amp selection; for expression in drosophila S2 cells; V5His tagged||||||Nicole
 +
|-
 +
| pFast-SpoCRY2.V5H||4/26/2012||Amp selection; make bacmids for Sf21 cells; V5His tagged||||||Nicole
 +
|-
 +
| CRY2-CX4 (pMal-c4x-SpoCRY2)||4/26/2012||Amp selection; for expression in BL21 or DH5a; MBP tagged||||||Nicole
 +
|-
 +
| Hm opsin 2||6/4/2012||||||||Nicole
 
|}
 
|}

Latest revision as of 14:50, 4 June 2012

Tube Label Date Notes Sequence Files Primers Used for Seq Lab Member
Vt10 10/12/2011 V. tsujii luciferase gene inserted in pcR2.1 vector; reversed FR 1F/6R Nicole
Vt12 10/12/2011 V. tsujii luciferase gene inserted in pcR2.1 vector; reversed FR , FR 1F/6R , 19/20 Nicole
Vt13 10/12/2011 V. tsujii luciferase gene inserted in pcR2.1 vector; reversed FR 1F/6R Nicole
pcDNA3 10/21/2011 pcDNA3 aliquot from Nick Doerr (Weimb's Lab) Nicole
Vt12/pcDNA3 1 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed FR 19/20 Nicole
Vt12/pcDNA3 2 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed FR 19/20 Nicole
Vt12/pcDNA3 3 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed FR 19/20 Nicole
Vt12/pcDNA3 4 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert Nicole
Vt12/pcDNA3 5 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert Nicole
Vt12/pcDNA3 6 12/9/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed Nicole
vt12_6 12/16/2011 V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; correct Nicole
Ligation 8/15 PhyVec, Ligation 8/15 Regrowth 8/15/2011 pHyVec1 derived plasmid: GFP, actin promoter, and actin polyadenylation site from hoTG trimmed and blunted at NsiI sites and cloned into Bluescript blunted at KpnI and SacI sites of MCS pHyVec13 Federico
pCR4-SpoCRY1-3 4/26/2012 Amp selection Nicole
pMalC2-SpoCRY1-3 4/26/2012 Amp selection; for expression in BL21 or DH5a; MBP tagged Nicole
pAC5.1-SpoCRY2.V5H 4/26/2012 Amp selection; for expression in drosophila S2 cells; V5His tagged Nicole
pFast-SpoCRY2.V5H 4/26/2012 Amp selection; make bacmids for Sf21 cells; V5His tagged Nicole
CRY2-CX4 (pMal-c4x-SpoCRY2) 4/26/2012 Amp selection; for expression in BL21 or DH5a; MBP tagged Nicole
Hm opsin 2 6/4/2012 Nicole
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