Difference between revisions of "Glycerol Stocks"
From Ucsbgalaxy
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| vt12_6||12/16/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; correct||||||Nicole | | vt12_6||12/16/2011||V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; correct||||||Nicole | ||
|- | |- | ||
| − | | ||||||||||Federico | + | | Ligation 8/15 PhyVec, Ligation 8/15 Regrowth||8/15/2011||pHyVec1 derived plasmid: GFP, actin promoter, and actin polyadenylation site from hoTG trimmed and blunted at NsiI sites and cloned into Bluescript blunted at KpnI and SacI sites of MCS ||[http://macroevolution.lifesci.ucsb.edu/IlluminaData/wikifiles/Sequences/pHyVec13.txt pHyVec13]||||Federico |
| + | |- | ||
| + | | pCR4-SpoCRY1-3||4/26/2012||Amp selection||||||Nicole | ||
| + | |- | ||
| + | | pMalC2-SpoCRY1-3||4/26/2012||Amp selection; for expression in BL21 or DH5a; MBP tagged||||||Nicole | ||
| + | |- | ||
| + | | pAC5.1-SpoCRY2.V5H||4/26/2012||Amp selection; for expression in drosophila S2 cells; V5His tagged||||||Nicole | ||
| + | |- | ||
| + | | pFast-SpoCRY2.V5H||4/26/2012||Amp selection; make bacmids for Sf21 cells; V5His tagged||||||Nicole | ||
| + | |- | ||
| + | | CRY2-CX4 (pMal-c4x-SpoCRY2)||4/26/2012||Amp selection; for expression in BL21 or DH5a; MBP tagged||||||Nicole | ||
| + | |- | ||
| + | | Hm opsin 2||6/4/2012||||||||Nicole | ||
|} | |} | ||
Latest revision as of 14:50, 4 June 2012
| Tube Label | Date | Notes | Sequence Files | Primers Used for Seq | Lab Member |
| Vt10 | 10/12/2011 | V. tsujii luciferase gene inserted in pcR2.1 vector; reversed | FR | 1F/6R | Nicole |
| Vt12 | 10/12/2011 | V. tsujii luciferase gene inserted in pcR2.1 vector; reversed | FR , FR | 1F/6R , 19/20 | Nicole |
| Vt13 | 10/12/2011 | V. tsujii luciferase gene inserted in pcR2.1 vector; reversed | FR | 1F/6R | Nicole |
| pcDNA3 | 10/21/2011 | pcDNA3 aliquot from Nick Doerr (Weimb's Lab) | Nicole | ||
| Vt12/pcDNA3 1 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed | FR | 19/20 | Nicole |
| Vt12/pcDNA3 2 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed | FR | 19/20 | Nicole |
| Vt12/pcDNA3 3 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed | FR | 19/20 | Nicole |
| Vt12/pcDNA3 4 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert | Nicole | ||
| Vt12/pcDNA3 5 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; no insert | Nicole | ||
| Vt12/pcDNA3 6 | 12/9/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; reversed | Nicole | ||
| vt12_6 | 12/16/2011 | V. tsujii luciferase gene (Vt12) inserted in pcDNA3 vector; correct | Nicole | ||
| Ligation 8/15 PhyVec, Ligation 8/15 Regrowth | 8/15/2011 | pHyVec1 derived plasmid: GFP, actin promoter, and actin polyadenylation site from hoTG trimmed and blunted at NsiI sites and cloned into Bluescript blunted at KpnI and SacI sites of MCS | pHyVec13 | Federico | |
| pCR4-SpoCRY1-3 | 4/26/2012 | Amp selection | Nicole | ||
| pMalC2-SpoCRY1-3 | 4/26/2012 | Amp selection; for expression in BL21 or DH5a; MBP tagged | Nicole | ||
| pAC5.1-SpoCRY2.V5H | 4/26/2012 | Amp selection; for expression in drosophila S2 cells; V5His tagged | Nicole | ||
| pFast-SpoCRY2.V5H | 4/26/2012 | Amp selection; make bacmids for Sf21 cells; V5His tagged | Nicole | ||
| CRY2-CX4 (pMal-c4x-SpoCRY2) | 4/26/2012 | Amp selection; for expression in BL21 or DH5a; MBP tagged | Nicole | ||
| Hm opsin 2 | 6/4/2012 | Nicole |
