Difference between revisions of "Antibody staining"
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(Created page with "Antibody list ('''updated July 20, 2012''') ''Primary antibodies:'' anti digoxigenin (sheep, monoclonal) anti digoxigenin-AP (sheep, polyclonal) ''Secondary fluorescent ant…") |
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''Primary antibodies:'' | ''Primary antibodies:'' | ||
| − | + | anti digoxigenin (sheep, monoclonal) | |
| − | + | anti digoxigenin-AP (sheep, polyclonal) | |
''Secondary fluorescent antibodies:'' | ''Secondary fluorescent antibodies:'' | ||
| − | + | Cy5 (?, anti-rabbit), ~650nm infrared | |
| − | + | Cy3 (?, anti-mouse), ~570nm tritc-ish | |
| − | + | Alexa 488 (mouse, anti-rabbit), 488nm fitc | |
| − | + | Alexa 488 (goat, anti-mouse), 488nm fitc | |
| − | + | Alexa 488 (donkey, anti-sheep), 488nm fitc | |
| − | + | Alexa 555 (?, anti-mouse), 555nm tritc | |
| − | + | Alexa 647 (donkey, anti-sheep), 647 infrared | |
''Scopes to visualize secondaries:'' | ''Scopes to visualize secondaries:'' | ||
Revision as of 11:54, 25 July 2012
Antibody list (updated July 20, 2012)
Primary antibodies:
anti digoxigenin (sheep, monoclonal)
anti digoxigenin-AP (sheep, polyclonal)
Secondary fluorescent antibodies:
Cy5 (?, anti-rabbit), ~650nm infrared
Cy3 (?, anti-mouse), ~570nm tritc-ish
Alexa 488 (mouse, anti-rabbit), 488nm fitc
Alexa 488 (goat, anti-mouse), 488nm fitc
Alexa 488 (donkey, anti-sheep), 488nm fitc
Alexa 555 (?, anti-mouse), 555nm tritc
Alexa 647 (donkey, anti-sheep), 647 infrared
Scopes to visualize secondaries:
LSB 4th floor compound fluorescent scope
DAPI
FITC
TRITC
Bio II 5th floor fluorescent confocal
DAPI
FITC
TRITC
Infrared
