Difference between revisions of "Antibody list"
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Alexa 647 (donkey, anti-sheep), 647 infrared | Alexa 647 (donkey, anti-sheep), 647 infrared | ||
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| + | """Other fluorescent dyes""" | ||
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| + | DAPI | ||
| + | FM1-43 (enters through TRP ion channels in mechanoreceptors, also used for endo/exocytosis) | ||
| + | Phalloidin 488 | ||
| + | A whole set of nuclear stains with different fluorophores | ||
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'''''Scopes to visualize secondaries:''''' | '''''Scopes to visualize secondaries:''''' | ||
Revision as of 12:15, 15 July 2013
Antibody list (updated July 25, 2012)
Primary antibodies:
anti digoxigenin (sheep, polyclonal, Roche)
anti digoxigenin-AP (sheep, polyclonal, Roche)
anti Gq (Drosophila) (rabbit, polyclonal, from )
anti opsin (Octopus) (rabbit, polyclonal, from Japanese company)
anti alpha tubulin (mouse, monoclonal)
anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7)
Secondary fluorescent antibodies:
Cy5 (?, anti-rabbit), ~650nm infrared
Cy3 (?, anti-mouse), ~570nm tritc-ish
Alexa 488 (mouse, anti-rabbit), 488nm fitc
Alexa 488 (goat, anti-mouse), 488nm fitc
Alexa 488 (donkey, anti-sheep), 488nm fitc
Alexa 555 (?, anti-mouse), 555nm tritc
Alexa 647 (donkey, anti-sheep), 647 infrared
"""Other fluorescent dyes"""
DAPI FM1-43 (enters through TRP ion channels in mechanoreceptors, also used for endo/exocytosis) Phalloidin 488 A whole set of nuclear stains with different fluorophores
Scopes to visualize secondaries:
LSB 4th floor compound fluorescent scope
DAPI
FITC
TRITC
Bio II 5th floor fluorescent confocal
DAPI
FITC
TRITC
Infrared
