Difference between revisions of "Antibody list"
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Alexa 647 (donkey, anti-sheep), 647 infrared | Alexa 647 (donkey, anti-sheep), 647 infrared | ||
| − | + | '''''Other fluorescent dyes''''' | |
DAPI | DAPI | ||
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FM1-43 (enters through TRP ion channels in mechanoreceptors, also used for endo/exocytosis) | FM1-43 (enters through TRP ion channels in mechanoreceptors, also used for endo/exocytosis) | ||
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Phalloidin 488 | Phalloidin 488 | ||
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A whole set of nuclear stains with different fluorophores | A whole set of nuclear stains with different fluorophores | ||
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'''''Scopes to visualize secondaries:''''' | '''''Scopes to visualize secondaries:''''' | ||
| − | LSB 4th floor compound fluorescent scope | + | ''LSB 4th floor compound fluorescent scope:'' |
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| − | FITC | + | DAPI, FITC, TRITC |
| − | + | ''Bio II 5th floor fluorescent confocal:'' | |
| − | Infrared | + | DAPI, FITC, TRITC, Infrared, DIC |
Latest revision as of 11:16, 15 July 2013
Antibody list (updated July 25, 2012)
Primary antibodies:
anti digoxigenin (sheep, polyclonal, Roche)
anti digoxigenin-AP (sheep, polyclonal, Roche)
anti Gq (Drosophila) (rabbit, polyclonal, from )
anti opsin (Octopus) (rabbit, polyclonal, from Japanese company)
anti alpha tubulin (mouse, monoclonal)
anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7)
Secondary fluorescent antibodies:
Cy5 (?, anti-rabbit), ~650nm infrared
Cy3 (?, anti-mouse), ~570nm tritc-ish
Alexa 488 (mouse, anti-rabbit), 488nm fitc
Alexa 488 (goat, anti-mouse), 488nm fitc
Alexa 488 (donkey, anti-sheep), 488nm fitc
Alexa 555 (?, anti-mouse), 555nm tritc
Alexa 647 (donkey, anti-sheep), 647 infrared
Other fluorescent dyes
DAPI
FM1-43 (enters through TRP ion channels in mechanoreceptors, also used for endo/exocytosis)
Phalloidin 488
A whole set of nuclear stains with different fluorophores
Scopes to visualize secondaries:
LSB 4th floor compound fluorescent scope:
DAPI, FITC, TRITC
Bio II 5th floor fluorescent confocal:
DAPI, FITC, TRITC, Infrared, DIC
