Difference between revisions of "Vargula tsujii"
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| + | ==Project History== | ||
We conducted 454 sequencing on bodies minus eyes. The specimens were collected from Catalina Island, with specific data available on the phylogeny website. | We conducted 454 sequencing on bodies minus eyes. The specimens were collected from Catalina Island, with specific data available on the phylogeny website. | ||
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There is also similarity to vitellogenein, through the VWD similarity. | There is also similarity to vitellogenein, through the VWD similarity. | ||
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| + | ==Project Notes and Ideas== | ||
| + | An interesting result is that a truncated luciferase from ''Vargula hilgendorfii'' produces bioluminescence, suggesting that one VWD domain is sufficient to catalyze the light reaction. | ||
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| + | <ref name="Horne">{{cite journal |author=D. J. Horne |url=http://www.cosis.net/abstracts/EGU2007/00093/EGU2007-J-00093.pdf?PHPSESSID=e |title=A Mutual Temperature Range Method for European Quaternary Nonmarine Ostracoda |journal=Geophysical Research Abstracts |volume=9 |pages=00093 |year=2007 |publisher=European Geosciences Union |format=[[Portable Document Format|PDF]]}}</ref> | ||
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| + | Maeda1996_JBiochem.pdf | ||
Revision as of 16:32, 17 November 2011
Project History
We conducted 454 sequencing on bodies minus eyes. The specimens were collected from Catalina Island, with specific data available on the phylogeny website.
I've made a galaxy history to assemble the transcriptome and look for luciferase genes [1]
There are 8 isotigs retrieved with an E-value of 0.001, when blasting the transcriptome with Vargula hilgendorfii luciferase. When blasted back to GenBank, all 8 of these have strong similarity to known ostracod luciferases.
These luciferase genes are also similar to kielin/crossveinless, which is involved in development.
There is also similarity to vitellogenein, through the VWD similarity.
Project Notes and Ideas
An interesting result is that a truncated luciferase from Vargula hilgendorfii produces bioluminescence, suggesting that one VWD domain is sufficient to catalyze the light reaction.
<ref name="Horne">Template:Cite journal</ref>
Maeda1996_JBiochem.pdf
