Difference between revisions of "Luciferase Assay"
From Ucsbgalaxy
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12/19/2011: Correct orientation insert and transfected lentivirus positive controls | 12/19/2011: Correct orientation insert and transfected lentivirus positive controls | ||
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| + | {| {{table}} | ||
| + | | align="center" style="background:#f0f0f0;"|'''Sample''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Well''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Type''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''Time''' | ||
| + | | align="center" style="background:#f0f0f0;"|'''CPS 10sec (CPS)''' | ||
| + | |- | ||
| + | | Negative control||A04||M||00:00:42.72||154 | ||
| + | |- | ||
| + | | Negative control||A05||M||00:00:58.13||173 | ||
| + | |- | ||
| + | | Negative control||A06||M||00:01:13.59||160 | ||
| + | |- | ||
| + | | Vector||B04||M||00:02:48.96||155 | ||
| + | |- | ||
| + | | Vector||B05||M||00:03:04.46||142 | ||
| + | |- | ||
| + | | Vector||B06||M||00:03:19.91||155 | ||
| + | |- | ||
| + | | Insert||A07||M||00:01:29.05||167 | ||
| + | |- | ||
| + | | Insert||A08||M||00:01:44.50||158 | ||
| + | |- | ||
| + | | Insert||A09||M||00:01:59.96||143 | ||
| + | |- | ||
| + | | Lentivirus positive control (10ul)||A10||M||00:02:15.40||5516696 | ||
| + | |- | ||
| + | | Lentivirus negative control||A11||M||00:02:30.88||8285 | ||
| + | |- | ||
| + | | Lentivirus negative control||B11||M||00:04:37.13||2442 | ||
| + | |- | ||
| + | | Lentivirus positive control||A10||M||00:02:15.40||5516696 | ||
| + | |- | ||
| + | | Lentivirus (5ul)||B07||M||00:03:35.34||210727 | ||
| + | |- | ||
| + | | Lentivirus (10ul)||B08||M||00:03:50.78||550716 | ||
| + | |- | ||
| + | | Lentivirus (15ul)||B09||M||00:04:06.24||837669 | ||
| + | |- | ||
| + | | Lentivirus (20ul)||B10||M||00:04:21.69||1033133 | ||
| + | |} | ||
| + | |||
| + | |||
12/02/2011: Lentivirus expressing Cyprindina luciferase (positive control) sent by Targeting Systems | 12/02/2011: Lentivirus expressing Cyprindina luciferase (positive control) sent by Targeting Systems | ||
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| Positive control||B03||M||00:02:01.36||2249091 | | Positive control||B03||M||00:02:01.36||2249091 | ||
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| − | |||
|} | |} | ||
Latest revision as of 12:35, 19 December 2011
12/19/2011: Correct orientation insert and transfected lentivirus positive controls
| Sample | Well | Type | Time | CPS 10sec (CPS) |
| Negative control | A04 | M | 00:00:42.72 | 154 |
| Negative control | A05 | M | 00:00:58.13 | 173 |
| Negative control | A06 | M | 00:01:13.59 | 160 |
| Vector | B04 | M | 00:02:48.96 | 155 |
| Vector | B05 | M | 00:03:04.46 | 142 |
| Vector | B06 | M | 00:03:19.91 | 155 |
| Insert | A07 | M | 00:01:29.05 | 167 |
| Insert | A08 | M | 00:01:44.50 | 158 |
| Insert | A09 | M | 00:01:59.96 | 143 |
| Lentivirus positive control (10ul) | A10 | M | 00:02:15.40 | 5516696 |
| Lentivirus negative control | A11 | M | 00:02:30.88 | 8285 |
| Lentivirus negative control | B11 | M | 00:04:37.13 | 2442 |
| Lentivirus positive control | A10 | M | 00:02:15.40 | 5516696 |
| Lentivirus (5ul) | B07 | M | 00:03:35.34 | 210727 |
| Lentivirus (10ul) | B08 | M | 00:03:50.78 | 550716 |
| Lentivirus (15ul) | B09 | M | 00:04:06.24 | 837669 |
| Lentivirus (20ul) | B10 | M | 00:04:21.69 | 1033133 |
12/02/2011: Lentivirus expressing Cyprindina luciferase (positive control) sent by Targeting Systems
| Sample | Well | Type | Time | CPS 10sec (CPS) |
| DAY 3 Negative control | A01 | M | 00:00:42.44 | 160 |
| DAY 3 Vector | A02 | M | 00:00:57.88 | 117 |
| DAY 3 Insert | A03 | M | 00:01:13.37 | 107 |
| Positive control | B01 | M | 00:01:30.45 | 2198944 |
| Positive control | B02 | M | 00:01:45.92 | 2220498 |
| Positive control | B03 | M | 00:02:01.36 | 2249091 |
