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− | Antibody list ('''updated July 20, 2012''') | + | [[Antibody list]] |
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− | '''''Primary antibodies:'''''
| + | [[Solution Recipes]] |
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− | anti digoxigenin (sheep, polyclonal, Roche)
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− | anti digoxigenin-AP (sheep, polyclonal, Roche)
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− | anti Gq (Drosophila) (rabbit, polyclonal, from )
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− | anti opsin (Octopus) (rabbit, polyclonal, from Japanese company)
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− | anti alpha tubulin (mouse, monoclonal)
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− | anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7)
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− | '''''Secondary fluorescent antibodies:'''''
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− | Cy5 (?, anti-rabbit), ~650nm infrared
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− | Cy3 (?, anti-mouse), ~570nm tritc-ish
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− | Alexa 488 (mouse, anti-rabbit), 488nm fitc
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− | Alexa 488 (goat, anti-mouse), 488nm fitc
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− | Alexa 488 (donkey, anti-sheep), 488nm fitc
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− | Alexa 555 (?, anti-mouse), 555nm tritc
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− | Alexa 647 (donkey, anti-sheep), 647 infrared
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− | '''''Scopes to visualize secondaries:'''''
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− | LSB 4th floor compound fluorescent scope
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− | DAPI
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− | FITC
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− | TRITC
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− | Bio II 5th floor fluorescent confocal
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− | DAPI
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− | FITC
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− | TRITC
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− | Infrared
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