Difference between revisions of "Opsin"
From Ucsbgalaxy
| Line 7: | Line 7: | ||
'''primer sequences:''' | '''primer sequences:''' | ||
| − | op.fout (forward outer): | + | op.fout (forward outer): 5' GCGGCATCAAGAAAATGTCC |
| − | op.rout (reverse outer): | + | op.rout (reverse outer): 5' CCATCATCGCTCTTCTTGCA |
length of PCR product: 427 bp | length of PCR product: 427 bp | ||
| − | |||
| − | reverse inner: | + | forward inner: 5' TCGGCCCCGTCTTCAACTGG |
| + | |||
| + | reverse inner: 5' ccagagcgctgaaatgaaac | ||
| + | |||
Latest revision as of 11:32, 2 May 2012
general primer info:
Made nested primers for opsin using O. bimaculoides opsin sequence [1] from Genbank.
primer sequences:
op.fout (forward outer): 5' GCGGCATCAAGAAAATGTCC
op.rout (reverse outer): 5' CCATCATCGCTCTTCTTGCA
length of PCR product: 427 bp
forward inner: 5' TCGGCCCCGTCTTCAACTGG
reverse inner: 5' ccagagcgctgaaatgaaac
qPCR primers
Also made shorter opsin primers suitable for qPCR using Primer3 website [2]
