Difference between revisions of "Antibody staining"
From Ucsbgalaxy
| Line 1: | Line 1: | ||
Antibody list ('''updated July 20, 2012''') | Antibody list ('''updated July 20, 2012''') | ||
| − | ''Primary antibodies:'' | + | '''''Primary antibodies:''''' |
anti digoxigenin (sheep, monoclonal) | anti digoxigenin (sheep, monoclonal) | ||
| Line 7: | Line 7: | ||
anti digoxigenin-AP (sheep, polyclonal) | anti digoxigenin-AP (sheep, polyclonal) | ||
| − | ''Secondary fluorescent antibodies:'' | + | '''''Secondary fluorescent antibodies:''''' |
Cy5 (?, anti-rabbit), ~650nm infrared | Cy5 (?, anti-rabbit), ~650nm infrared | ||
| Line 23: | Line 23: | ||
Alexa 647 (donkey, anti-sheep), 647 infrared | Alexa 647 (donkey, anti-sheep), 647 infrared | ||
| − | ''Scopes to visualize secondaries:'' | + | '''''Scopes to visualize secondaries:''''' |
LSB 4th floor compound fluorescent scope | LSB 4th floor compound fluorescent scope | ||
Revision as of 11:55, 25 July 2012
Antibody list (updated July 20, 2012)
Primary antibodies:
anti digoxigenin (sheep, monoclonal)
anti digoxigenin-AP (sheep, polyclonal)
Secondary fluorescent antibodies:
Cy5 (?, anti-rabbit), ~650nm infrared
Cy3 (?, anti-mouse), ~570nm tritc-ish
Alexa 488 (mouse, anti-rabbit), 488nm fitc
Alexa 488 (goat, anti-mouse), 488nm fitc
Alexa 488 (donkey, anti-sheep), 488nm fitc
Alexa 555 (?, anti-mouse), 555nm tritc
Alexa 647 (donkey, anti-sheep), 647 infrared
Scopes to visualize secondaries:
LSB 4th floor compound fluorescent scope
DAPI
FITC
TRITC
Bio II 5th floor fluorescent confocal
DAPI
FITC
TRITC
Infrared
