Difference between revisions of "Antibody staining"

Revision as of 12:17, 25 July 2012

Antibody list (updated July 25, 2012)

Primary antibodies:

anti digoxigenin (sheep, polyclonal, Roche)

anti digoxigenin-AP (sheep, polyclonal, Roche)

anti Gq (Drosophila) (rabbit, polyclonal, from )

anti opsin (Octopus) (rabbit, polyclonal, from Japanese company)

anti alpha tubulin (mouse, monoclonal)

anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7)

Secondary fluorescent antibodies:

Cy5 (?, anti-rabbit), ~650nm infrared

Cy3 (?, anti-mouse), ~570nm tritc-ish

Alexa 488 (mouse, anti-rabbit), 488nm fitc

Alexa 488 (goat, anti-mouse), 488nm fitc

Alexa 488 (donkey, anti-sheep), 488nm fitc

Alexa 555 (?, anti-mouse), 555nm tritc

Alexa 647 (donkey, anti-sheep), 647 infrared

Scopes to visualize secondaries:

LSB 4th floor compound fluorescent scope

DAPI

FITC

TRITC

Bio II 5th floor fluorescent confocal

DAPI

FITC

TRITC

Infrared