Difference between revisions of "G-q alpha"
| Line 7: | Line 7: | ||
'''primer sequences:''' | '''primer sequences:''' | ||
| − | + | Gq.fout (forward outer): 5' ctwgggactggygagtctgg | |
| − | + | Gq.rout (reverse outer): 5' cgaatggargartcaaaagc | |
length of PCR product: 630 bp | length of PCR product: 630 bp | ||
| − | forward inner: | + | forward inner: 5' CAGATGAGAATTATCCATGG |
| − | reverse inner: | + | reverse inner: 5' CGTCCATCATGTTTTTAGTM |
| Line 22: | Line 22: | ||
Also made shorter opsin primers suitable for qPCR using Primer3 website [http://frodo.wi.mit.edu/] | Also made shorter opsin primers suitable for qPCR using Primer3 website [http://frodo.wi.mit.edu/] | ||
| − | + | Other Gq primer sets that I made but never used: | |
| − | |||
| − | |||
| − | |||
| − | |||
gproaO | gproaO | ||
forward outer 5' CAGGAATTCGGCACAGTAGG | forward outer 5' CAGGAATTCGGCACAGTAGG | ||
Latest revision as of 20:47, 15 November 2012
general primer info:
Made nested primers for G protein alpha q using E. scopoles and O. vulgaris sequences from Genbank
primer sequences:
Gq.fout (forward outer): 5' ctwgggactggygagtctgg
Gq.rout (reverse outer): 5' cgaatggargartcaaaagc
length of PCR product: 630 bp
forward inner: 5' CAGATGAGAATTATCCATGG
reverse inner: 5' CGTCCATCATGTTTTTAGTM
qPCR primers
Also made shorter opsin primers suitable for qPCR using Primer3 website [1]
Other Gq primer sets that I made but never used: gproaO forward outer 5' CAGGAATTCGGCACAGTAGG inner 5' AAAGAAGATGGTATACAAGC reverse inner 5' cgaactagagttaagacaac outer 5' ggtcaaagatcggaacgtaa gproI forward outer 5' tgtwtacagcaacatwatgc inner 5' gatgaawgtgacatgactcc
reverse inner 5' ggatccactgyttygaaggtg outer 5' gctatwatcttcattgtggc
