Difference between revisions of "Antibody staining"
| Line 7: | Line 7: | ||
anti digoxigenin-AP (sheep, polyclonal, Roche) | anti digoxigenin-AP (sheep, polyclonal, Roche) | ||
| − | anti Gq (Drosophila) ( | + | anti Gq (Drosophila) (rabbit, polyclonal, from ) |
anti opsin (Octopus) (rabbit, polyclonal, from Japanese company) | anti opsin (Octopus) (rabbit, polyclonal, from Japanese company) | ||
| + | |||
| + | anti alpha tubulin (mouse, monoclonal) | ||
| + | |||
| + | anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7) | ||
'''''Secondary fluorescent antibodies:''''' | '''''Secondary fluorescent antibodies:''''' | ||
Revision as of 12:15, 25 July 2012
Antibody list (updated July 20, 2012)
Primary antibodies:
anti digoxigenin (sheep, polyclonal, Roche)
anti digoxigenin-AP (sheep, polyclonal, Roche)
anti Gq (Drosophila) (rabbit, polyclonal, from )
anti opsin (Octopus) (rabbit, polyclonal, from Japanese company)
anti alpha tubulin (mouse, monoclonal)
anti beta tubulin (mouse, monoclonal, from Iowa Hybridoma Bank, E7)
Secondary fluorescent antibodies:
Cy5 (?, anti-rabbit), ~650nm infrared
Cy3 (?, anti-mouse), ~570nm tritc-ish
Alexa 488 (mouse, anti-rabbit), 488nm fitc
Alexa 488 (goat, anti-mouse), 488nm fitc
Alexa 488 (donkey, anti-sheep), 488nm fitc
Alexa 555 (?, anti-mouse), 555nm tritc
Alexa 647 (donkey, anti-sheep), 647 infrared
Scopes to visualize secondaries:
LSB 4th floor compound fluorescent scope
DAPI
FITC
TRITC
Bio II 5th floor fluorescent confocal
DAPI
FITC
TRITC
Infrared
