Difference between revisions of "'Scrunch' assay"
From Ucsbgalaxy
(Created page with "# Starve Hydra for at least 48 hours. # Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM. #…") |
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| + | Purpose: Test for photosensitivity | ||
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# Starve Hydra for at least 48 hours. | # Starve Hydra for at least 48 hours. | ||
# Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM. | # Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM. | ||
| − | # Turn on light at desired intensity and wait until | + | # Turn on light at desired intensity and wait until scrunch response or 20 minutes. |
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| + | References: | ||
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| + | This was used to test for CNG involvement in photobehavior in Plachetzki et al 2010 [http://rspb.royalsocietypublishing.org/content/early/2010/03/02/rspb.2009.1797.full] | ||
Revision as of 13:04, 15 November 2011
Purpose: Test for photosensitivity
- Starve Hydra for at least 48 hours.
- Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM.
- Turn on light at desired intensity and wait until scrunch response or 20 minutes.
References:
This was used to test for CNG involvement in photobehavior in Plachetzki et al 2010 [1]
