Difference between revisions of "'Scrunch' assay"
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Purpose: Test for photosensitivity | Purpose: Test for photosensitivity |
Revision as of 21:59, 18 November 2011
Purpose: Test for photosensitivity
- Starve Hydra for at least 48 hours.
- Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM.
- Turn on light at desired intensity and wait until scrunch response or 20 minutes.
References:
This was used to test for CNG involvement in photobehavior in Plachetzki et al 2010 [1]