Difference between revisions of "Opsin"

Revision as of 11:01, 2 May 2012

general primer info:

Made nested primers for opsin using O. bimaculoides opsin sequence [1] from Genbank.

primer sequences:

op.fout (forward outer):

op.rout (reverse outer):

length of PCR product: 427 bp

forward inner:

reverse inner:

qPCR primers

Also made shorter opsin primers suitable for qPCR using Primer3 website [2]

@@ Line 1: / Line 1: @@
-primer info:
+'''general primer info:'''
+Made nested primers for opsin using ''O. bimaculoides'' opsin sequence [http://www.ncbi.nlm.nih.gov/nuccore/AY545172.1] from Genbank.
+'''primer sequences:'''
+op.fout (forward outer):
+op.rout (reverse outer):
+length of PCR product: 427 bp
+forward inner:
+reverse inner:
+'''qPCR primers'''
+Also made shorter opsin primers suitable for qPCR using Primer3 website [http://frodo.wi.mit.edu/]