'Scrunch' assay
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Revision as of 13:52, 18 November 2011 by 128.111.226.247 (talk)
Purpose: Test for photosensitivity
- Starve Hydra for at least 48 hours.
- Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM.
- Turn on light at desired intensity and wait until scrunch response or 20 minutes.
References:
This was used to test for CNG involvement in photobehavior in Plachetzki et al 2010 [1]