Opsin
From Ucsbgalaxy
Revision as of 11:32, 2 May 2012 by 128.111.226.191 (talk)
general primer info:
Made nested primers for opsin using O. bimaculoides opsin sequence [1] from Genbank.
primer sequences:
op.fout (forward outer): 5' GCGGCATCAAGAAAATGTCC
op.rout (reverse outer): 5' CCATCATCGCTCTTCTTGCA
length of PCR product: 427 bp
forward inner: 5' TCGGCCCCGTCTTCAACTGG
reverse inner: 5' ccagagcgctgaaatgaaac
qPCR primers
Also made shorter opsin primers suitable for qPCR using Primer3 website [2]
