Antibody staining

Antibody list (updated July 20, 2012)

Primary antibodies:

anti digoxigenin (sheep, monoclonal)

anti digoxigenin-AP (sheep, polyclonal)

Secondary fluorescent antibodies:

 Cy5 (?, anti-rabbit), ~650nm infrared

 Cy3 (?, anti-mouse), ~570nm tritc-ish

 Alexa 488 (mouse, anti-rabbit), 488nm fitc

 Alexa 488 (goat, anti-mouse), 488nm fitc

 Alexa 488 (donkey, anti-sheep), 488nm fitc

 Alexa 555 (?, anti-mouse), 555nm tritc

 Alexa 647 (donkey, anti-sheep), 647 infrared

Scopes to visualize secondaries:

LSB 4th floor compound fluorescent scope

DAPI

FITC

TRITC

Bio II 5th floor fluorescent confocal

DAPI

FITC

TRITC

Infrared