'Scrunch' assay

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  1. Starve Hydra for at least 48 hours.
  2. Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM.
  3. Turn on light at desired intensity and wait until scruch response or 20 minutes.