G-q alpha

general primer info:

Made nested primers for G protein alpha q using E. scopoles and O. vulgaris sequences from Genbank

primer sequences:

op.fout (forward outer):

op.rout (reverse outer):

length of PCR product: 630 bp

forward inner:

reverse inner:

qPCR primers

Also made shorter opsin primers suitable for qPCR using Primer3 website [1]

gproaQ forward outer 5' ctwgggactggygagtctgg inner 5' CAGATGAGAATTATCCATGG reverse inner 5' CGTCCATCATGTTTTTAGTM outer 5' cgaatggargartcaaaagc gproaO forward outer 5' CAGGAATTCGGCACAGTAGG inner 5' AAAGAAGATGGTATACAAGC reverse inner 5' cgaactagagttaagacaac outer 5' ggtcaaagatcggaacgtaa gproI forward outer 5' tgtwtacagcaacatwatgc inner 5' gatgaawgtgacatgactcc

reverse inner 5' ggatccactgyttygaaggtg outer 5' gctatwatcttcattgtggc