G-q alpha

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general primer info:

Made nested primers for G protein alpha q using E. scopoles and O. vulgaris sequences from Genbank


primer sequences:

Gq.fout (forward outer): 5' ctwgggactggygagtctgg

Gq.rout (reverse outer): 5' cgaatggargartcaaaagc

length of PCR product: 630 bp

forward inner: 5' CAGATGAGAATTATCCATGG

reverse inner: 5' CGTCCATCATGTTTTTAGTM


qPCR primers

Also made shorter opsin primers suitable for qPCR using Primer3 website [1]

Other Gq primer sets that I made but never used: gproaO forward outer 5' CAGGAATTCGGCACAGTAGG inner 5' AAAGAAGATGGTATACAAGC reverse inner 5' cgaactagagttaagacaac outer 5' ggtcaaagatcggaacgtaa gproI forward outer 5' tgtwtacagcaacatwatgc inner 5' gatgaawgtgacatgactcc

reverse inner 5' ggatccactgyttygaaggtg outer 5' gctatwatcttcattgtggc

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