'Scrunch' assay

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Purpose: Test for photosensitivity

  1. Starve Hydra for at least 48 hours.
  2. Isolate a single Hydra in a 15mL dish and place in the dark for half an hour in 10 mL of HM. If doing a drug trail, add it to the HM.
  3. Turn on light at desired intensity and wait until scrunch response or 20 minutes.


References:

This was used to test for CNG involvement in photobehavior in Plachetzki et al 2010 [1]

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