Opsin
From Ucsbgalaxy
Revision as of 11:01, 2 May 2012 by 128.111.226.191 (talk)
general primer info:
Made nested primers for opsin using O. bimaculoides opsin sequence [1] from Genbank.
primer sequences:
op.fout (forward outer):
op.rout (reverse outer):
length of PCR product: 427 bp
forward inner:
reverse inner:
qPCR primers
Also made shorter opsin primers suitable for qPCR using Primer3 website [2]
