G-q alpha

general primer info:

Made nested primers for G protein alpha q using E. scopoles and O. vulgaris sequences from Genbank

primer sequences:

op.fout (forward outer):

op.rout (reverse outer):

length of PCR product: 630 bp

forward inner:

reverse inner:

qPCR primers

Also made shorter opsin primers suitable for qPCR using Primer3 website [1]