Opsin

general primer info:

Made nested primers for opsin using O. bimaculoides opsin sequence [1] from Genbank.

primer sequences:

op.fout (forward outer): 5' GCGGCATCAAGAAAATGTCC

op.rout (reverse outer): 5' CCATCATCGCTCTTCTTGCA

length of PCR product: 427 bp

forward inner: 5' TCGGCCCCGTCTTCAACTGG

reverse inner: 5' ccagagcgctgaaatgaaac

qPCR primers

Also made shorter opsin primers suitable for qPCR using Primer3 website [2]