Pouring an agarose gel

Use gloves!

50mL buffer ~0.5g agarose 1 uL ethidium bromide

1. Mix agarose into buffer and heat until all agarose is incorporated.
2. Wait until mixture is cool enough to hold comfortably in hand then add ethidium bromide
3. Rotate glass jar gently then cool
4. Place rubber stoppers on the ends of the plastic channel
5. Insert comb to form wells, rounded edges up
6. Make sure the combs are at least 1mm away from the bottom
7. Pour agarose mixture into channel
8. Pour buffer into dish below the gel channel
9. Wait until cool (gel will become cloudy)
10. Place Ladder into a well as a standard size (5uL)
11. Mix loading buffer (1 uL) with the sample you are running (total ~5uL) on parafilm and # load
12. Turn meter on to ~100 Volts
13. Align channel so that the + side is farther from the samples
14. Let run until sample bands are about 1/2 way down the gel