Difference between revisions of "Nematocyte firing"
From Ucsbgalaxy
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==Background== | ==Background== | ||
| − | David Plachetzki discovered that phototransduction gene expression is associated with nematocyte cells. We hypothesized that light may modulate their firing, in addition to other sensory modalities which are already known to modulate firing. Dave and Caitlin Fong then developed experiments to test whether light modulates firing. They conducted [[Nematocyte Capture]] assays under different light intensities of blue light in Hydra, and found significant differences. | + | * David Plachetzki discovered that phototransduction gene expression is associated with nematocyte cells. We hypothesized that light may modulate their firing, in addition to other sensory modalities which are already known to modulate firing. Dave and Caitlin Fong then developed experiments to test whether light modulates firing. They conducted [[Nematocyte Capture]] assays under different light intensities of blue light in Hydra, and found significant differences. |
| − | Katia's thesis gives more extensive background about this [[Katia Jindrich 2011]]. | + | * Katia's thesis gives more extensive background about this [[Katia Jindrich 2011]]. |
| − | Another important source is Dave's original paper [[Plachetzki nematocyte firing]]. | + | * Another important source is Dave's original paper [[Plachetzki nematocyte firing]]. |
Revision as of 17:32, 18 November 2011
Background
- David Plachetzki discovered that phototransduction gene expression is associated with nematocyte cells. We hypothesized that light may modulate their firing, in addition to other sensory modalities which are already known to modulate firing. Dave and Caitlin Fong then developed experiments to test whether light modulates firing. They conducted Nematocyte Capture assays under different light intensities of blue light in Hydra, and found significant differences.
- Katia's thesis gives more extensive background about this Katia Jindrich 2011.
- Another important source is Dave's original paper Plachetzki nematocyte firing.
Unpublished Experiments
- Katia visited for 6 months and conducted firing experiments in Haliplanella using combinations of light and mucin. Katia's thesis is here Katia Jindrich 2011.
- Federico visited for 6 months and conducted experiments in Nematostella to test combinations of G-protein inhibiting drug, light, and amino acids.
- Dan did MSP on the Haliplanella symbionts to find that the absorbance peak is similar to an opsin peak.
Next Step Experiments
- Adam aims to establish the behavioral action spectrum for nematocyte modulation. This involves measuring the nematocyte capture rate under different colors of light, but with standardized light intensity.
