Nematocyte Capture

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PROBING

  1. . Starve animals at least 48 hours but no more than 72.
  2. . Place 15 Hydra in a 20 mL dish and add 15 mL HM.
  3. . Adapt Hydra to correct light intensity and wavelength for 6 hours.
  4. . Probe and immediately count discharged nematocysts (recommended 40x).

MAKING PROBES

  1. . Cut fishing line (4 lb).
  2. . Coat in Lysine (adds stickiness)
  3. . Dip 3 times in Jello.
  4. . Let dry between 30 minutes and 1 hour.
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